Study of the week.

A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cells.

Ngai PH, Ng TB.

Biochem Biophys Res Commun. 2004 Feb 20;314(4):988-93. 

Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.

An 18-kDa lectin, with an N-terminal sequence displaying slight similarity to some lectins and fungal immunomodulatory proteins, was isolated from the mushroom Ganoderma capense (Lloyd) Teng. It exhibited more potent mitogenic activity than that of concanavalin A toward mouse splenocytes, and antiproliferative activity toward leukemia (L1210 and M1) cells and hepatoma (HepG2) cells. The isolation procedure entailed ion exchange chromatography on Q-Sepharose, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 75. D(+)-galactose and D(+)-galactosamine specifically inhibited the hemagglutinating activity of the lectin. The hemagglutinating activity of the lectin was not affected over the temperature range 0-100 degrees C and after exposure to 100 degrees C for 60min. The activity was stable in the pH range of 4-11, and after incubation with solutions of various chlorides (from 3.125 to 50mM) including NaCl, KCl, CaCl(2), MgCl(2), ZnCl(2), MnCl(2), and AlCl(3). However, it was potentiated by 12.5-50mM FeCl(3). The lectin was devoid of HIV-1 reverse transcriptase inhibitory and antifungal activities.

MeSH Terms:

• Amino Acid Sequence
• Animals
• Cell Division/*drug effects
• Cell Line, Tumor
• Chromatography, Gel
• Chromatography, Ion Exchange
• Electrophoresis, Polyacrylamide Gel
• Ganoderma/*chemistry
• Lectins/chemistry/isolation & purification/*pharmacology
• Mice
• Mitogens/chemistry/isolation & purification/*pharmacology
• Molecular Sequence Data
• Sequence Homology, Amino Acid
• Spleen/cytology/*drug effects

Substances:

• 0 (Lectins)
• 0 (Mitogens)

Publication Status: ppublish
PMID: 14751230 [PubMed - indexed for MEDLINE]

• Ganoderma Science Links

  • Another link from the National Institute of Health This about suppressing HIV!
  • Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms.
  • Anticancer Effects of Ganoderma lucidum: A Review of Scientific Evidence
  • Article from The Encyclopedia of Alternative Medicine A good overview.
  • Effects of Ganoderma lucidum on Human Leukoeytes Another abstract from a study about ganoderma and leukemia.
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